High-yield, automated intracellular electrophysiology in retinal pigment epithelia
نویسندگان
چکیده
منابع مشابه
The intercellular junctional complexes of retinal pigment epithelia.
Previous studies of the retinal pigment epithelium have suggested that its cells are interconnected by typical arrays of intercellular junctions. Using both conventional and freezefracture electron microscopy, we have found on the contrary that pigment epithelial cells in a wide variety of vertebrates (goldfish, frog, newt, mudpuppy, turtle, chicken, mouse, cat, and monkey) in fact have unusual...
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Electrophysiological measurements, in particular, patch clamping, have long been regarded as the "gold standard" for assaying ion channels. Despite its high information content, the technique suffers from laborious, manual processing by highly skilled workers and extremely low throughput. Recently, a number of researchers have started to automate patch clamping by either automating conventional...
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Myopia is a common ocular disorder generally due to increased axial length of the eye-globe. Its extreme form high myopia (HM) is a multifactorial disease leading to retinal and scleral damage, visual impairment or loss and is an important health issue. Mutations in the endocytic receptor LRP2 gene result in Donnai-Barrow (DBS) and Stickler syndromes, both characterized by HM. To clearly establ...
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Monocarboxylate transporters (MCTs) are a family of highly homologous membrane proteins that mediate the 1:1 transport of a proton and a lactate ion. In chicken, MCT3 is preferentially expressed in the retinal pigment epithelium (RPE). We have isolated the mouse MCT3 cDNA and gene and characterized the pattern of tissue expression. MCT3 is a single copy gene with a 1.8-kb transcript that encode...
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C O N T E N T S 1. In t roduc t ion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 1.1. M o r p h o l o g y of Ret inal Glial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 1.1.1. Miil ler cells . . . . . . . . . . . . . . . . . . . ...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2019
ISSN: 0165-0270
DOI: 10.1016/j.jneumeth.2019.108442